Fig. 2

A. The impact of 24 h of CTEP or rapamycin exposure upon α-syn aggregation in SH-SY5Y cells previously exposed to α-syn PFFs (fib) for a six-day period. A significant reduction of α-syn positive fibril particle area was observed with both the CTEP and rapamycin (rap) treatments in response to the α-syn fibrils alone or in combination with the A53T AV. The A53T AV alone increased overall cellular fluorescence (in the absence of obvious intracellular particles) and this was also reduced by CTEP and rapamycin. B. Quantified data were derived from separate experiments (n = 9/group) with each carried out in triplicate. (* p < 0.05, difference compared to no A53T AV; # p < 0.05, difference compared to vehicle controls (ctrl); @ p < 0.05, difference compared to α-syn PFF (fib) treatment in the absence of CTEP or rap). Particle count was performed with Image J and statistical analysis: SPSS. Tukey’s pairwise comparison was for post hoc comparisons. Scale bars: 100 μm