Fig. 3

The mTOR pathway was affected by CTEP and rapamycin treatments alone and in the context of α-syn fibril exposure. A. The ratio of phosphorylated, p-mTOR, to total, mTOR, was taken as an index of mTOR activation. The CTEP and rapamycin (rap) treatments both reduced p-mTOR/mTOR protein levels, relative to either vehicle treated controls (ctrl) or treatment with α-syn fibril (fib) ± A53T AV exposure. B. The α-syn fibril + A53T AV combination treatment increased p-AKT/AKT levels above controls and this was reduced by both CTEP and rapamycin treatments. C. The α-syn fibril + A53T AV treatment combination increased p-p70s6 protein levels, relative to vehicle treated controls and this was markedly reduced by both the CTEP and rapamycin treatments. (* p < 0.05, difference compared to vehicle control (ctrl); # p < 0.05, difference compared α-syn fibril (fib) alone treatment). Presented data are the result of three separate experiments (n = 3/group) with each carried out in triplicate. All blot signals were normalized to total protein content. Following significant ANOVAs, Tukey’s pairwise comparison was used for post hoc comparisons