Fig. 2

Long-term ODC1 KD switches astrocytes from reactive to active state in APP/PS1 mice. A Representative images of proBDNF and GFAP-immunoreactive cells in the hippocampus of virus-injected APP/PS1 animals (Scale bar 40 μm, white dashed line represents the interface between CA1 layer lacunosum moleculare and molecular layer of DG). B Bar graph representing the intensity of proBDNF in the GFAP-positive cells from images in panel A (Ordinary one-way ANOVA with Sidak’s multiple comparisons test). C Average number of intersections of the astrocytic branches with concentric circles in Sholl analysis from images in panel A (RM two-way ANOVA with Geisser-Greenhouse correction with Tukey’s multiple comparisons test; *p value for WTScr vs TgScr; #p value for WTScr vs TgODC1; no significant differences between TgScr and TgODC1). D Bar graph representing the ending radius of concentric circles around astrocyte branches in Sholl analysis (Ordinary one-way ANOVA with Tukey’s multiple comparisons test). E Bar graph representing the sum of intersections made by the astrocyte branches and concentric circles in Sholl analysis (Ordinary one-way ANOVA with Tukey’s multiple comparisons test). Data represents Mean ± SEM. # or*p < 0.05, ## or **p < 0.01,### or ***p < 0.001, #### or ****p < 0.0001