Fig. 3

Transcriptome of long-term ODC1-inhibited astrocytes shows dramatic changes, resembling enriched environment-house animal brains. A Experimental timeline schematic for bulk RNA sequencing of primary astrocyte culture. B Volcano plot representing the differential expression of specific genes associated with astrocyte reactivity, learning and memory, apoptosis, transcriptional regulation, ubiquitination and autophagy from bulk RNA-Seq. Horizontal dotted line refers to p < 0.05, vertical dotted line indicates no change in expression level). C Volcano plots representing the differential expression of genes involved in different GO terms from the bulk-RNASeq dataset. Horizontal dotted line refers to p < 0.05, vertical dotted line indicates no change in expression level. D Gene ontology analysis for synapse-associated genes from bulk RNA-Seq of Aβ and Aβ + DFMO-treated primary cultured astrocytes. E Gene ontology analysis for histone modification-associated genes from bulk RNA-Seq of Aβ and Aβ + DFMO-treated primary cultured astrocytes. F Gene ontology analysis for protein processing pathways and related genes from bulk RNA-Seq of Aβ and Aβ + DFMO-treated primary cultured astrocytes. G Gene ontology analysis for genes associated with apoptosis from bulk RNA-Seq of Aβ and Aβ + DFMO-treated primary cultured astrocytes