Fig. 2

Prenatally VPA exposure leads to abnormal synaptic development in offspring rats (A) PSD-95 mRNA expression (n = 3 for each group); (B) SYN mRNA expression (n = 3 for each group); (C) Gephyrin mRNA expression (n = 3 for each group); (D) PSD-95 protein expression (n = 3 for each group); (E) SYN protein expression (n = 3 for each group); (F) Gephyrin protein expression (n = 3 for each group); (G) Protein bands of PSD-95, SYN and Gephyrin (n = 3 for each group); (H) Synaptic cleft (n = 3 for each group); (I) Thickness of postsynaptic dense substance (n = 3 for each group); (J) Length of postsynaptic dense substance (n = 3 for each group); (K) Synaptic transmission electron micrograph, ⇧synapse, ↑ presynaptic membrane, △ synaptic cleft, ▲ postsynaptic dense substance. Data are expressed as mean ± SD. Data were analyzed using the student’s t-test. Compared with the same age control group, *P < 0.05; Compared with PND 7 in the same group, #P < 0.05