Fig. 3

Prenatally VPA exposure changes the phenotype of microglia in offspring rats (A) IL-1β (n = 3 for each group); (B) IL-4 (n = 3 for each group); (C) Expression of CD86 mRNA (n = 3 for each group); (D) Expression of CD206 mRNA (n = 3 for each group); (E) Expression of TREM2 mRNA (n = 3 for each group); (F) Expression of DAP12 mRNA (n = 3 for each group); (G) Expression of CD86 protein (n = 3 for each group); (H) Expression of CD206 protein (n = 3 for each group); (I) Expression of TREM2 protein (n = 3 for each group); (J) Expression of DAP12 protein (n = 3 for each group); (K) Protein bands of CD86, CD206, TREM2 and DAP12. Data are expressed as mean ± SD. Data were analyzed using the student’s t-test. Compared with the same age control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; Compared with PND 7 in the same group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001