Fig. 5

TREM2 regulates microglia function through P38 MAPK pathway (A) After TREM2 interference, TREM2 mRNA expression (n = 3 for each group); (B) After TREM2 interference, TREM2 protein expression (n = 3 for each group); (C) After TREM2 overexpression, TREM2 mRNA expression (n = 3 for each group); (D) After TREM2 overexpression, TREM2 protein expression (n = 3 for each group); (E) Protein bands of TREM2 interference and overexpression; (F) IL-1β (n = 3 for each group); (G) IL-4 (n = 3 for each group); (H) PSD-95 protein expression (n = 3 for each group); (I) SYN protein expression (n = 3 for each group); (J) Gephyrin protein expression (n = 3 for each group); (K) Protein bands of PSD-95, SYN and Gephyrin; (L) CD86 protein expression (n = 3 for each group); (M) CD206 protein expression (n = 3 for each group); (N) TREM2 protein expression (n = 3 for each group); (O) p-P38 MAPK protein expression (n = 3 for each group); (P) P38 MAPK protein expression (n = 3 for each group); (Q) p-ELK-1 protein expression (n = 3 for each group); (R) ELK-1 protein expression (n = 3 for each group); (S) Protein bands of CD86, CD206, TREM2, p-P38 MAPK, P38 MAPK, p-ELK-1 and ELK-1. Data are expressed as mean ± SD. Data were analyzed using the F-test and t-test. Compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; Compared with the ASD group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001