Fig. 4

Effects of a single administration of hNB001 on nociception, motor, and anxiety-like behavior in mice. a, b There was no significant difference in hind paw withdrawal to von Frey filaments (a) and response latency of the hot plate test (b) after a single oral administration of 10 mg/kg hNB001 in adult mice, compared with the saline group (Mechanical withdrawal, saline, n = 10 mice, hNB001, n = 10 mice, Student’s t-test, t ₍₁₈₎ = 0.2689, p = 0.7911; Hot plate, saline, n = 8 mice, hNB001, n = 9 mice, Student’s t-test, t ₍₁₅₎ = 1.651, p = 0.1195). c There was no significant difference in motor performance between hNB001- and saline-treatment adult mice (Saline, n = 10 mice, hNB001, n = 10 mice, Student’s t-test, t ₍₁₈₎ = 0.5280, p = 0.6040). d The two squares on the left are representative traces showing the movement of hNB001- and saline-treatment mice in the open field test. The pink box is the central area and the green is the peripheral area. There was no significant difference in motor performance and anxiety-related behavior of the open field test after a single oral administration of hNB001 in adult mice, compared with the saline group (Saline, n = 10 mice, hNB001, n = 10 mice, Student’s t-test, total distance, t ₍₁₈₎ = 0.6008, p = 0.5555; Time in center, t ₍₁₈₎ = 0.2326, p = 0.8187; Number of center entries, t ₍₁₈₎ = 0.4884, p = 0.6311). e, f There was no significant difference in hind paw withdrawal to von Frey filaments (e) and response latency of the hot plate test (f) after a single oral administration of 10 mg/kg hNB001 in aged mice, compared with the saline group (Mechanical withdrawal, saline, n = 9 mice, hNB001, n = 10 mice, Student’s t-test, t ₍₁₇₎ = 1.328, p = 0.2017; Hot plate, saline, n = 8 mice, hNB001, n = 8 mice, Student’s t-test, t ₍₁₄₎ = 0.8348, p = 0.4179). g There was no significant difference in motor performance between oral administration of hNB001 and saline in aged mice (Saline, n = 10 mice, hNB001, n = 10 mice, Student’s t-test, t ₍₁₈₎ = 1.058, p = 0.3039). h The two squares on the left are representative traces showing the movement of hNB001- and saline-treatment mice in the open field test. The pink box is the central area and the green is the peripheral area. There was no significant difference in motor performance and anxiety-related behavior of the open field test after a single oral administration of hNB001 in aged mice, compared with the saline group (Saline, n = 9 mice, hNB001, n = 8 mice, Student’s t-test, total distance, t ₍₁₅₎ = 0.8076, p = 0.4319; Time in center, t ₍₁₅₎ = 1.390, p = 0.1862; Number of center entries, t ₍₁₅₎ = 1.363, p = 0.1959)