Fig. 5
Effects of continuous administration of hNB001 on weight, nociception, motor, and anxiety-like behaviors in mice. a Continuous oral administration of 10 mg/kg hNB001 did not affect the weight of adult mice (Saline, n = 10 mice; hNB001, n = 10 mice, Two-way ANOVA, F (1,18) = 0.0152, p = 0.9031). b, c Continuous oral administration of hNB001 did not affect hind paw withdrawal to von Frey filaments (b) and response latency of the hot plate test (c) in adult mice (Saline, n = 8 mice, hNB001, n = 10 mice, mechanical withdrawal, Student’s t-test, t (16) = 0.1582, p = 0.8762; Hot plate, Student’s t-test, t (16) = 0.7155, p = 0.4846). d Continuous oral administration of hNB001 did not affect motor performance in adult mice (Saline, n = 8 mice, hNB001, n = 10 mice, Student’s t-test, t (16) = 1.460, p = 0.1638). e The two squares on the left are representative traces showing the movement of hNB001- and saline-treatment mice in the open field test. The pink box is the central area and the green is the peripheral area. Continuous oral administration of hNB001 did not affect motor performance and anxiety-related behavior of the open field test in adult mice (Saline, n = 8 mice, hNB001, n = 10 mice, Student’s t-test, total distance, t (16) = 0.6770, p = 0.5081; Time in center, t (16) = 0.1241, p = 0.9028; Number of center entries, t (16) = 0.7239, p = 0.4796). f Continuous oral administration of 10 mg/kg hNB001 did not affect the weight of aged mice (Saline, n = 8 mice; hNB001, n = 10 mice, Two-way ANOVA, F (1,16) = 0.1211, p = 0.7324). g, h Continuous oral administration of hNB001 did not affect hind paw withdrawal to von Frey filaments (g) and response latency of the hot plate test (h) in aged mice (Saline, n = 8 mice, hNB001, n = 10 mice, mechanical withdrawal, Student’s t-test, t (16) = 0.7895, p = 0.4413; Hot plate, Student’s t-test, t (16) = 0.4190, p = 0.6808). i Continuous oral administration of hNB001 did not affect motor performance in aged mice (Saline, n = 8 mice, hNB001, n = 10 mice, Student’s t-test, t (16) = 1.539, p = 0.1434). j The two squares on the left are representative traces showing the movement of hNB001- and saline-treatment mice in the open field test. The pink box is the central area and the green is the peripheral area. Continuous oral administration of hNB001 did not affect motor performance and anxiety-related behavior of the open field test in aged mice (Saline, n = 8 mice, hNB001, n = 10 mice, Student’s t-test, total distance, t (16) = 0.9894, p = 0.3372; Time in center, t (16) = 0.2430, p = 0.8113; Number of center entries, t (16) = 0.1349, p = 0.8944)