Fig. 4
From: Spermidine treatment: induction of autophagy but also apoptosis?
Immunoblotting of wild-type and transgenic CMVMJD135 mice confirms increased expression of full-length ataxin-3 in the cerebellum of 18-week-old MJD mice. (A) Representative western blot of 18-week-old wild-type and MJD mouse cerebellum following oral vehicle or spermidine supplementation confirming expression of full-length and endogenous ataxin-3. (B) Quantification of full-length ataxin-3 expression shows significant increase in the cerebellum of 18-week-old MJD mice compared to wild-type mice (p < 0.0001, n = 6–7). Expression of full-length ataxin-3 is not significantly altered following vehicle or spermidine treatment. A two-way ANOVA was utilised for statistical analysis followed by Tukey post hoc. Data represents means ± SEM. *** Represents p < 0.0001