Fig. 2
From: Mutant α-synuclein causes death of human cortical neurons via ERK1/2 and JNK activation
PD patient-derived cortical neurons show shorter neurite length and time-dependent vulnerability. A Representative images used for quantification of neurite length on day 9 after neuronal induction using the IncuCyte ZOOM imaging system. Green and blue represent identified neurites and cell body clusters, respectively. Scale bar = 200 μm. B Quantification data of neurite length on day 9 after neuronal induction. Neurite lengths of PD patient-derived cortical neurons are compared to control-derived cortical neurons (n = 3 biological replicates; two-tailed Student’s t-test; *p < 0.05). C Representative images of caspase-3/7 positive cells (green) on day 9, day 12, and day 15 after neuronal induction with or without Z-VAD-FMK, caspase-3/7 inhibitor. Scale bar = 400 μm. zVAD: Z-VAD-FMK. D Quantification data of caspase-3/7 positive cells on day 9, day 12, and day 15 after neuronal induction. PD patient-derived cortical neurons are compared to control-derived cortical neurons (n = 3 biological replicates; two-tailed Student’s t-test; N.S. not significant, *p < 0.05, **p < 0.01). Bar graphs represent mean ± SD