Fig. 3
From: Mutant α-synuclein causes death of human cortical neurons via ERK1/2 and JNK activation
RNA-sequencing analysis implies activation of MAPK cascades in PD patient-derived cortical neurons. A Sample-to-sample distance heatmap of variance stabilized expression data for all six lines (CTL#1, CTL#2, CTL#3, PD#1, PD#2-1, PD#2-2) of cortical neurons on day 8 and two lines (CTL#1, PD#1) of iPSCs. The darker grey colors indicate more similar transcriptome profiles. B Hierarchical clustering heatmap showing differentially expressed genes (DEGs) between PD patient-derived and control-derived cortical neurons on day 8. Z-scores are used to compare expression levels between samples. Red bands and blue bands indicate higher expression and lower expression, respectively. C Gene ontology (GO) enrichment analysis using Metascape. Bar charts showing the top 15 GO terms among statistically significant biological processes. D Expression levels of the genes identified from RNA sequencing analysis that is differentially expressed and constitute the gene ontology (GO) term “positive regulation of MAPK cascade (GO:0043410)”. The x-axis represents the ratio of the average values of PD patient-derived cortical neurons to that of control-derived cortical neurons. E Volcano plots visualizing transcription factors that could regulate the DEGs predicted by ChIP-Atlas. Each dot represents an individual SRX ID