Fig. 5

Enhanced freezing responses of Nlgn3^mf mutant mice in the contextual fear conditioning with a 0.5 mA electric shock. Freezing responses during conditioning with footshocks of 0.5 mA (a, e), contextual test-1 (b, f), extinction session (c, g), and contextual-test 2 (d, h) of the Nlgn3^hse mutant mice and their littermate WT mice (a–d) and of the Nlgn3^mf mutant mice and their littermate WT mice (e–h) are quantified. Arrows represent footshock. There were no significant differences in freezing responses between the Nlgn3^hse mutant mice and their littermate WT mice, while the Nlgn3^mf mutant mice exhibited significantly higher freezing responses than their littermate WT mice during conditioning, extinction session, and contextual-test 2. Summary statistics are shown in each panel. All values are presented as the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001, Two-tailed t-test in e, g, h, and paired two-tailed t-test in d, h