Dose-titration binding constants of RO25,6981, ifenprodil and haloperidol performed on Buffer -9 refolded 6 × His-ATD2B protein determined from CD. Values are mean ± S.E. of the KD values from five experiments using proteins obtained from at least two independent batches of bacteria induction. Rat NR1 and NR2B subunits were coexpressed in Xenopus oocytes. NR2B-selective antagonists' inhibition of current responses induced by glutamate and glycine at pH7.3 were analyzed to obtain the mean-fitted IC50 values with n representing number of oocytes recorded.