Figure 1

Intracellular application of Abl decreases NMDA-evoked currents. A) Active (black square) or heat-inactivated (denat., white square) Abl kinase (0.5 μg/mL) was included in the intracellular solution. NMDA-evoked currents were recorded once every minute and peak currents were normalized to the average of the first five currents recorded. At 25 minutes (b) Abl significantly reduced NMDA-evoked currents (p < 0.05, Student's t-test, n = 6). B, C) Representative currents from currents recorded in the presence of active Abl kinase or heat-inactivated kinase at t = 3 minutes (a) or t = 25 minutes (b). D) NMDA-evoked currents were recorded with active Abl kinase applied intracellularly in the ICF in the absence (black square) or presence (black triangle) of the Abl kinase inhibitor, STI571, applied in the ECF bath (n = 6). E) NMDA-evoked currents were recorded with active Abl kinase applied intracellularly in the ICF in the absence (black square) or presence (black triangle) of the Abl kinase inhibitor, STI571, applied in the ECF bath for 25 minutes (STI571), followed by a 25 minute washout period (WO) during which control ECF solution was applied (n = 6).