Figure 4

The ROCK inhibitor, Y-27632, attenuates Abl kinase-induced inhibition of NMDA evoked currents. A) Abl kinase (black triangle) or control (white square) was included in the intracellular solution. NMDA-evoked currents were recorded once every minute and peak currents were normalized to the average of the first five currents recorded (n = 7).). At 25 minutes Abl significantly reduced NMDA-evoked currents (p < 0.05, Student's t-test, n = 5), B) NMDA-evoked peak currents were recorded every minute in the presence of 1 μM Y-27632 with control (ROCK inhibitor, white square) or Abl kinase (+Abelson, black triangle) included in the intracellular solution. Peak currents were normalized to the average of the first five currents recorded (n = 5). C) Slices were homogenized and the lysates were immunoprecipitated with an anti-ROCK antibody. Lysates and immunoprecipitates were resolved via SDS-Page electrophoresis and immunoblotted with anti-phospho-tyrosine (PY) or anti-ROCK antibodies. Blots are representative of 4 experiments. D) Slices were homogenized and the lysates were immunoprecipitated with an anti-Abl antibody. Lysates and immunoprecipitates were resolved via SDS-Page electrophoresis and immunoblotted with anti-WAVE1 antibody. Blot is representative of 4 experiments.