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Table 1 The effect of PDGF-BB on the subcellular fractionation of PDGFβ receptors, ROCK, and NR1

From: Abelson tyrosine kinase links PDGFbeta receptor activation to cytoskeletal regulation of NMDA receptors in CA1 hippocampal neurons

Protein Change in localization after PDGF-BB treatment
  S2 S3 P3  
PDFGβ receptor n/a 63 ± 15%a 136 ± 31%  
ROCK 60 ± 12%a 66 ± 30% 125 ± 30%  
NR1 n/a 81 ± 36% 130 ± 30%  
  1. Hippocampal slices were treated for 10 minutes with 10 ng/mL PDGF-BB, were homogenized, and S2, S3, and P3 fractions were prepared as described in the methods section. Equal amounts of total protein from each fraction were resolved on SDS gels, transferred to nitrocellulose membranes, and immunoblotted with anti- PDFGβ receptor (n = 4), anti-ROCK (n = 3), or anti-NR1 (n = 3) antibodies. Data represent the mean +/- standard error, a p < 0.05, Student's unpaired t-test.