Figure 2

Specific Abnormalities in the DG of Alpha-CaMKII+/- Mice. (A, B) Dopamine D1 receptor binding was highly increased in the DG of alpha-CaMKII+/- mice. (C, D) NMDA receptor binding was similarly downregulated in the DG and CA1 of alpha-CaMKII+/- mice. (E-J) Among the downregulated genes in the hippocampus of alpha-CaMKII+/- mice, several genes were expressed selectively in the DG (Allen Brain Atlas [23] [Internet]. Seattle, WA: Allen Institute for Brain Science. © 2004–2008. Available from: http://www.brain-map.org.). Graphs indicate the relative expression levels of each gene in the microarray experiment, which are confirmed by quantitative RT-PCR (see Additional file 1, Figure S6). (K, L) c-Fos expression following electric foot shock was selectively decreased in the DG of mutant mice at the age of 8 weeks. BLA, basolateral amygdaloid nucleus; MeA, medial amygdaloid nucleus; LimCX, limbic cortex; Ent, entorhinal cortex; CPU, caudate nucleus/putamen; NAC, nucleus accumbens; SNr, substantia nigra pars reticulata; CA1rad, stratum radiatum of CA1; CA1or, stratum oriens of CA1; MDT, mediodorsal thalamic nucleus; CTX, cingulated cortex; SEP, septum; OrCX, orbital cortex; TGN, tegmental nuclei; CB, cerebellum; Ncx, neocortex; LH, lateral hypothalamus; CeA, central amygdaloid nucleus. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Error bars indicate s.e.m.