Figure 2
Maintenance of rotarod-running skill requires protein synthesis. (A) Naïve rats were trained one trial each day for 3 consecutive days. Anisomycin or ACSF was infused bilaterally into the lateral ventricles 6 h pre-trial. An additional ANI infusion was performed on day 4, with no training after the infusion. Rats treated with anisomycin (ANI) performed the rotarod-running task equally well with those treated with ACSF in the 3 training days. The performance level of ANI-treated rats dropped dramatically in the day-5 retention test, but was recovered in the day-6 retention trial. * p < 0.05, for ANI group (n = 9 rats) vs. ACSF group (n = 12 rats), unpaired t-test. ## p < 0.01, for ANI group on day 3 vs. day 5, paired t-test. Data are expressed as means ± SEM. (B) Naïve rats were trained one trial each day for 3 consecutive days. Anisomycin or ACSF was infused bilaterally into the lateral ventricles 6 h pre-trial. An additional ANI infusion was performed on day 5, 6 hours prior to the retention test. Rats treated with anisomycin (ANI) performed the rotarod-running task equally well with those treated with ACSF in the 3 training days. The performance level of ANI-treated rats was similar to that of the ACSF group in the day-5 and day-6 retention trials. ANI group, n = 9 rats; ACSF group, n = 12 rats. Data are expressed as means ± SEM. (C) Naïve rats were trained one trial each day for 7 consecutive days. Surgery was performed on day 4 after the last training trial. Rats were retrained one trial on day 4 after the surgery. Anisomycin (ANI) or ACSF was administered into the lateral ventricles 2 days post-retraining, as indicated by the black arrow, and were tested one trial each day for the subsequent 2 days. The two groups performed the task equally well in the last training trial and did so in the post-surgery re-training trial. However, the anisomycin group exhibited a deficient performance in the 1st testing trial. The deficient performance was recovered in the 2nd testing trial. * p < 0.05 for ANI group (n = 7 rats) vs. ACSF group (n = 7 rats), unpaired t-test; # p < 0.05 for post-infusion trial vs. pre-infusion trial, paired t-test. Data are expressed as means ± SEM. Right bottom: A representative placement of the guide cannuale for anisomysin infusion. The trace for the injection needle could be identified clearly. LV: lateral ventricle.