Skip to main content
Figure 2 | Molecular Brain

Figure 2

From: Molecular genetic analysis of FGFR1 signalling reveals distinct roles of MAPK and PLCγ1 activation for self-renewal of adult neural stem cells

Figure 2

A chimeric receptor recapitulates effects of FGF-2 and implicates Erk1/2 and PLCγ1 signalling in adult NSC self-renewal. (A) A schematic diagram illustrating the chimeric receptor and the amino acid residues within the FGFR1 intracellular domain that are linked to various downstream signalling pathways. Shown on the top is the RT-PCR analysis of the expression of endogenous TrkA and FGFRs under the proliferating condition in the presence of FGF-2. (B) A bright-field view (low and high density) of NSC lines with the TF1 chimeric receptor grown in the presence of FGF-2 (20 ng/ml) or the surrogate ligand NGF (50 ng/ml). Scale bar: 20 μm. (C) Growth curves of the NSC lines with TF1 chimeric receptors in the absence, and presence of FGF-2 (20 ng/ml) or the surrogate ligand NGF (50 ng/ml). Values represent mean ± SEM. (n = 3). (D) Quantification of NSC numbers of various chimeric receptor lines cultured in the medium alone, or with supplementation of FGF-2 (20 ng/ml) or NGF (50 ng/ml). For each line, the total NSC numbers were normalized to those cultures in the presence of FGF-2 (20 ng/ml). Values represent mean ± SEM. (n = 3; *: P < 0.01, Student's t-test). (E) Normal NSC characteristics and multipotentiality of the TF1 chimeric NSC line maintained by NGF. Shown on the top are sample images of immunostaining of proliferating NSCs with Ki67 (red) and Nestin (green). Shown on the bottom are sample images of immunostaining with Tuj1 (red) or GFAP (green) of cultures at 6 days after the treatment of RA (0.5 μM) and FBS (0.5%). Scale bar: 20 μm.

Back to article page