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Figure 4 | Molecular Brain

Figure 4

From: Molecular genetic analysis of FGFR1 signalling reveals distinct roles of MAPK and PLCγ1 activation for self-renewal of adult neural stem cells

Figure 4

Erk1/2 Activation is both required and sufficient for adult NSC proliferation. (A) Western blot analysis of Erk1/2 inhibition in normal adult NSCs with the treatment of U0126 or U0124 at different concentrations. (B) Quantification of the cellular composition of adult NSC culture in the presence or absence of U0126, or its inactive analog U0124 (2.5 μM). Values represent mean ± SEM. (n = 6; *: P < 0.01, Student's t-test). (C) Sample images of clonal analysis of adult NSCs with MEK1 inhibition. Scale bar: 20 μm. (D) Summary of clonal analysis of adult NSCs in the presence of U0126 or U0124. (E) Western blot analysis of Erk1/2 activation in MEK1-WT, DN or CA lines. (F) Summary of clonal analysis assay for adult NSCs expressing MEK1-DN, WT or CA. Values represent mean ± SEM. (n = 3; *: P < 0.01, Student's t-test).

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Molecular Brain

ISSN: 1756-6606