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Figure 1 | Molecular Brain

Figure 1

From: A novel mechanism of hippocampal LTD involving muscarinic receptor-triggered interactions between AMPARs, GRIP and liprin-α

Figure 1

Properties of CCh-induced LTD in the CA1 region of the hippocampus. (A) A pooled data (n = 8) of EPSC amplitude vs time to show that carbachol application (CCh, 50 μM, 10 min) induces mAChR-LTD. (B) D-AP5 (50 μM) has no effect on mAChR-LTD (n = 9). (C) mAChR-LTD was prevented by bath application of an M1 mAChR antagonist, pirenzepine (0.5 μM, n = 5). (D) The M1 specific agonist, 77-LH-28-1, induces LTD (n = 6). (E) D-AP5 (50 μM) has no effect on LTD induced by 77-LH-28-1 (n = 5). (F) Pirenzepine (0.5 μM) prevents LTD induced by 77-LH-28-1 (n = 7). (G) Co-application of LY367385 (100 μM) and MPEP (50 μM) has no effect on mAChR-LTD (n = 6). (H) An example of biotinylation from hippocampal slices. Pooled data (n = 4) shows that CCh induces internalisation of GluA2. Error bars represent s.e.m.

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Molecular Brain

ISSN: 1756-6606