Figure 3

PTZ induced seizure increased the expression of cleaved caspases-3 in prenatal rat hippocampal neurons. Western blot analyses of the caspases-3 in the primary cultured hippocampal neuronal cells at GD 17.5 from PTZ-induced seizure model during pregnancy. Cells are exposed to different drugs for 20 min as previously explained. Immunoblotting with polyclonal antibody showed processing of caspases-3 with the appearance of active fragment (17 kDa cleaved caspase-3). Detail procedures are mentioned in material and method section. β-actin is taken as loading control in each case. A: Immunoblots of caspases-3 of hippocampal neuronal cells under different treatment conditions. The immunoblots were labeled with an anti caspases-3 antibody B: Density values were expressed as mean ± SEM (n = 4, mean four rat per group) of the corresponding protein of caspases-3 are presented. The density values on (Y-axis) are expressed as arbitrary units (AU). *P < 0.05 and **P < 0.01 versus control group.