Figure 4

PTZ-induced seizure induced apoptotic neurodegeneration. (a): Propidium iodide (PI) and Fluoro-jade B staining analysis of the neurodegeneration in prenatal rat hippocampal from PTZ-induced seizure models. Detail procedures are mentioned in material and method section. These histological sections are from prenatal rat at GD 17.5 days-old to a vehicle-treated mother. Maternal exposure of PTZ during pregnancy induces neurodegeneration in the hippocampal area of fetal rat brain (A-F). Interruptions in the CA1 lamination, clear neurodegeneration and change in cell morphology were observed in prenatal fetal of pregnant rat treated with PTZ. The photograph documented that in prenatal rat brain from PTZ-induced seizure model has triggered a robust neurodegeneration reaction throughout many regions of the fetal rat hippocampal areas, whereas saline has left the brain showing only a sparse of apoptotic degeneration attributable to physiological cell death that occurs normally in the developing brain. Panels (B, E = ×100 and C, F = ×40) are magnified views from panels (A, D = ×20), representing PI and FJB staining in hippocampal area. (b): Representing the co treatment of FJB and PI staining in hippocampal area of brain. Magnification 100×, Scale bar = 10 μm.