Decreased motility of Rab5-associated endosomes in ALS2-/- neurons. A-E. Representative kymographs of GFP-Rab5-positive endosome movement in wild-type (WT (Rab5), A), ALS2-/- (KO (Rab5), B), Rab5Q-transfected wild-type (WT (Rab5Q), C), DsRed-ALS2 transfected ALS2-/- (KO (Rab5+ALS2), D), and DsRed-vector alone transfecetd ALS2-/- (KO (Rab5+vector), E) hippocampal neurons. Each kymograph summarizes the spatial positions (x-axis) of Rab5 endosomes at certain time points (y-axis) in one video field. F. Histogram shows the average speed of the Rab5-positive endosomes (n > 30) in WT (Rab5), KO (Rab5), WT (Rab5Q), KO (Rab5+ALS2), and KO (Rab5+vector) neurons. Error bars indicate SEM. **P < 0.05. ***P < 0.01.