Increased co-localization of LAMP1 with Rab5-associated vesicles in ALS2-/- neurons. (A-D) A lower (left) or higher magnification of Rab5-transfected wild-type (WT, A), or ALS2-/- (KO, B) neurons co-stained with LAMP1 (red) and GFP (green) antibodies. Co-localization of LAMP1 (red) and Rab5 (green) in WT and KO neurons was revealed by z-series stack images. Scale bars = 20 μm (left) and 10 μm (the rest) respectively. C. Quantitative analysis of co-localization of LAMP1 and Rab5 immuno-reactivity in WT (n = 4) and KO (n = 7) neurons. Error bars indicate SEM. * P < 0.05. D-E. In the soma, LAMP1 (red) and Rab5Q (green) were co-localized in either GFP-Rab5Q-transfected wild-type (WT, D) or ALS2-/- (KO, E) neurons. Scale bar = 20 μm.