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Figure 1 | Molecular Brain

Figure 1

From: cGMP-dependent protein kinase Iα associates with the antidepressant-sensitive serotonin transporter and dictates rapid modulation of serotonin uptake

Figure 1

Colocalization of SERT and PKGI in RN46A cells. A) Total RNA from RN46A cells or rat midbrain was isolated and subjected to RT-PCR as described in Methods. Figure shows agarose gel analysis of cDNA products, revealing expression of PKGI but very little PKGII. B-E) Double immunocytochemical labeling of SERT, PKGI, and PKGII. RN46A cells were fixed, stained, and imaged by confocal microscopy as described in Methods. SERT staining was achieved with an ectodomain antibody (Advanced Targeting AB-N09). NP, nonpermeabilized. P, permeabilized by 0.2% NP40. NP/B, nonpermeabilized with antibody preabsorbtion by peptide (Advanced Targeting PR-03). DIC image is inset in B, middle, to demonstrate presence of cells. Inset image in D, right, shows staining in process. Closed arrows indicate perinuclear staining while open arrows indicate process staining. Scale bars represent 10 μm. Data are representative of at least three independent experiments.

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Molecular Brain

ISSN: 1756-6606