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Table 1 Bioluminescence data from control animals

From: A riot of rhythms: neuronal and glial circadian oscillators in the mediobasal hypothalamus

Tissue

n

% Rhythmic

Period (hours)

Phase (hours after cull)

Amplitude (arbitrary units)

Rate of Damping (days)

Camera

      

DMH (whole)

12

100

21.9 ± 0.8

28.6 ± 0.6

2247 ± 775

3.0 ± 0.4

DMH (single cells)

131

81.7

22.4 ± 0.3

28.4 ± 1.3

2483 ± 400

†

Arc (whole)

12

100

23.1 ± 0.3

28.6 ± 0.3

3658 ± 1128

5.2 ± 0.6

Dorsal Arc (single cells)

129

89.1

22.6 ± 0.6

28.6 ± 0.6

5805 ± 2221

†

Lateral Arc (single cells)

97

67.0

22.6 ± 0.4

28.5 ± 0.6

2854 ± 812

†

Ependymal

12

92

22.3 ± 0.5

30.4 ± 1.3

1168 ± 234

3.6 ± 0.5

ME/PT

12

100

23.3 ± 0.4

31.3 ± 0.9

1901 ± 740

6.9 ± 1.0

SCN (whole)

4

100

24.1 ± 0.3

9.4 ± 0.8^

14622 ± 5835#

∞

SCN (single cells)

40

97.5

23.6 ± 0.1

8.0 ± 0.2^

21444 ± 1297#

∞

PMT

      

Whole MBH

11

100

22.8 ± 0.6

29.4 ± 0.7

325 ± 61

3.3 ± 0.2

DMH

13

100

24.2 ± 0.5

30.6 ± 0.7

170 ± 21

2.6 ± 0.2

Arc/ME/PT

10

100

23.5 ± 0.6

30.5 ± 0.7

169 ± 31

3.1 ± 0.3

SCN

6

100

23.9 ± 0.2

9.8 ± 0.2^

5426 ± 1215

∞

  1. ^ phase for SCN cultures is in Zeitgeber time, not time from cull, as SCN rhythms are not reset by cull/culture procedure.
  2. † not all single cells had damped completely by the end of imaging; ~80% of cells were still clearly oscillating after one week.
  3. # values not directly comparable to data for MBH regions as different detection settings were used to image SCN cultures due to their much higher levels of bioluminescence (see experimental procedures/results).