Figure 6
From: Cocaine induces cell death and activates the transcription nuclear factor kappa-b in pc12 cells
(A) Cocaine (COC) exposure decreased Bcl-2 protein expression in PC12 cells. Cell lysates were extracted from PBS and COC (1 mM, 6 hours) treated PC12 cells. Cell lysate protein content (30 μg) was loaded and run on a SDS-polyacrylamide gel and submitted to Immunoblot assay. The densitometric analysis (% of control) of the Bax and Bcl-2 band complexes represented in (A) are shown (B and C). β-Actin was used as internal control. (D) Effect of incubation with COC (1 mM) for 6 or 24 hours on levels of mRNA for Bax, Bcl-2 and BDNF in PC12 cells. Cocaine increased BDNF mRNA levels after 6 hours (G) and decreased Bcl-2 mRNA levels after 6 and 24 hours of cocaine treatment (F) as indicated by RT-PCR assays. No changes were observed for Bax mRNA levels after both 6 and 24 hours of cocaine treatment (E). Results are expressed as mean + S.E.M. from three individual experiments. * p < 0.05 vs PBS (one-way ANOVA followed by Newman-Keuls test).