Figure 1

FK506 increases huntingtin phosphorylation at S421 in primary cultures of mouse Hdh^Q111/Q111 cortical neurons. A) 3 DIV cortical primary neurons were treated with DMSO or different FK506 concentrations (0.1 μM, 0.3 μM and 1 μM) for 1 hour and processed for Western blotting analysis with anti-phospho-huntingtin-S421-714, anti-huntingtin mAb4C8 or anti-α-tubulin antibodies. (B) Graph represents the quantitative assessment of the optical density of phosphorylated huntingtin on three independent experiments and is expressed as the phospho-huntingtin/total huntingtin ratio normalised to total huntingtin. O.D. ratios, DMSO versus FK506 0.1 μM and 0.3 μM (**p < 0.01), FK506 1 μM (**p < 0.001).