Figure 5

Silencing of calcineurin by RNA interference in cortical neurons from Hdh^Q111/Q111 mutant mice restores BDNF transport. A) Cortical primary HD neurons were electroporated with BDNF-mCherry plasmid and siRNAs against the isoforms α and β of the calcineurin A subunit. 48 h after electroporation, half of the neurons were lysed and extracts were analyzed by western blot for siRNA efficiency and huntingtin phosphorylation and, the other half of neurons were analyzed by videomicroscopy (B-D). (B and C) Calcineurin depletion increase significantly anterograde (**p < 0.01) and retrograde (**p < 0.01) velocity of BDNF vesicles. (D) Pausing time was reduced (**p < 0.01) in this mutant mice. Data are from two independent experiments, 5550 tracks, 20 cells for scramble RNA and 3461 tracks, 16 cells for siRNA calcineurin in Hdh^Q111/Q111 neurons.