Figure 3
From: CBP/p300 is a cell type-specific modulator of CLOCK/BMAL1-mediated transcription
The NR and KIX domains of CBP are required for the repression of CLOCK/BMAL1 activity. (A), (C) NIH3T3 cells were transiently transfected with pE-box (2 ng), together with or without pcDNA3CLOCK (100 ng) plus pcDNA3BMAL1 (100 ng) in combination with pcDNA3CBP (100 ng) or pCMV-HA carrying the indicated CBP deletion mutants (1-1098, 1-312, 313-1098, 1099-2441, 1099-1867, 1868-2441, 185-1098, Δ KIX, 1-711; 100 ng), as indicated. Empty vector (pcDNA3) was used to standardize the total amount of transfected DNA (402 ng). An asterisk indicates p < 0.05 compared with lane 2 (Student's t test). (B), (D) Protein expression of wild-type CBP and CBP mutants was verified by Western blotting of NIH3T3 cell lysates using HA-antibody. α-tubulin serves as an equal loading control.