Table 1 Parameters for Buffered Ca2+ Diffusion Models
Quantity | Immature (P8-12) | Mature (P16-18) | Reference |
|---|---|---|---|
ATP Diffusion Constant | 220 μm2/s | 220 μm2/s | 57 |
Kd (ATP) | 2300 μM | 2300 μM | 57 |
kon (ATP) | 500 μM-1•s-1 | 500 μM-1•s-1 | 57 |
[ATP]pipette | 2000 μM | 2000 μM | 57 |
EGTA Diffusion Constant | 220 μm2/s | 220 μm2/s | 57 |
Kd (EGTA) | 0.18 μM | 0.18 μM | 57 |
kon (EGTA) | 2.5 μM-1•s-1 | 2.5 μM-1•s-1 | 57 |
[EGTA]pipette | 500/10000 μM | 500 μM | 36 |
BAPTA Diffusion Constant | 220 μm2/s | 220 μm2/s | 57 |
Kd (BAPTA) | 0.22 μM | 0.22 μM | 57 |
kon (BAPTA) | 400 μM-1•s-1 | 400 μM-1•s-1 | 57 |
[BAPTA]pipette | 1000 μM | 1000 μM | 36 |
ENDO Diffusion Constant | 15 μm2/s | 15 μm2/s | 57 |
Kd (ENDO) | 50 μM | 50 μM | 57 |
kon (ENDO) | 100 μM-1•s-1 | 100 μM-1•s-1 | 57 |
[ENDO]pipette | 500 μM | 500 μM | 57 |
Ca2+ Diffusion Constant | 220 μm2/s | 220 μm2/s | 57 |
Resting [Ca2+]i | 50 nM | 50 nM | 58 |
Steady-State Single VGCC Flux | 6.24 × 105 ions/s | 6.24 × 105 ions/s | 24 |