Effects of suppressing Rho and Rac activity in staurosporine-induced cell death. (A) Anti-activated caspase-3 and anti-ssDNA immunostaining of cultured NSPCs infected with recombinant adenoviruses expressing both RhoDN and RacDN. Higher proportions of anti-activated caspase-3 or ssDNA immunopositive cells (red) were observed in the control condition (arrows). Several GFP-negative cells expressed active caspase-3 (arrow heads). Cells were counterstained with Hoechst 33342 dye to visualize nuclei (blue). Bar, 20 μm. (B) Quantification of immunopositive cells for anti-activated caspase-3 or anti-ssDNA antibodies colocalized with GFP. Reduction of immunopositive cells was statistically significant (anti-activated caspase-3; p < 0.01, anti-ssDNA; p < 0.01, Mann-Whitney test).