Figure 8From: Critical involvement of Rho GTPase activity in the efficient transplantation of neural stem cells into the injured spinal cordEnhancement of cell survival in the injured spinal cord by suppression of Rho and Rac activity. (A) Distribution of transplanted stem cells expressing both RhoDN and RacDN 7 days after transplantation. Cells expressing both RhoDN and RacDN (green) survived in the injured spinal cord (arrow). The sections were stained with anti-neurofilament-200 antibody (red). Arrowhead indicates the site of transection. Bar, 200 μm. (B) Quantification of cells present in the injured spinal cords 7 days after transplantation of NSPCs expressing RhoDN and RacDN. There was a significant increase of surviving cell numbers after transplantation of DN forms of Rho and Rac (control; 179.7 ± 28.6 cells (n = 6), RhoDN and RacDN; 420.6 ± 72.2 cells (n = 5), t-test p < 0.01).Back to article page