Reduced eye size and disrupted lamination in Chx10Cre-RBPf/f retinae. (A) Schematic illustration depicting the genetic scheme used to generate Chx10Cre-RBPf/fmice. (B) P21 Chx10Cre-RBPf/fmice show reduced eye size relative to wild-type controls. (C, D) HE staining of P21 retinal sections shows three nuclear layers and two synaptic layers are distinctly recognizable in wild-type mice (C), but the lamination is severely distorted with many rosettes in Chx10Cre-RBPf/fretina (D). (E-J) Nissl staining of wild-type (E, G, I) and Chx10Cre-RBPf/fretinae (F, H, J) at E12.5 (E, F), E13.5 (G, H), and E14.5 (I, J). Note that the rosettes are first detected at E13.5. (K-N) The adjacent sections processed respectively for HE and X-gal staining indicate that the clustered X-gal+ (mutant) cells (arrows) are located within the rosettes in Pet1Cre-RBPf/fretinae (L, N). Images from Pet1Cre retinae are shown for comparison (K, M). O and P are high magnification views of (M) and (N), respectively. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer. Scale bar, C-N, 100 μm, O, P, 50 μm.