Up-regulation of ganglion cell differentiation-related genes and increase of ganglion cells in early retinogenesis of Chx10Cre-RBPf/fmice. (A, B) Expression of the proneural gene Math5 is increased in RBP-J deficient retinae compared to wild-type retinae at E12.5. (C-F) The expression domains of Nefl and islet1, two ganglion cell markers, are enlarged in RBP-J deficient retinae (D, F) compared with wild-type controls at E13.5 (C, E). (G-L) Immunostaining for Brn3 reveals an increase in the number of ganglion cells at E13.5 (G, H) and E16.5 (I, J), but a decrease at P0 (K, L) in RBP-J deficient retinae (H, J, L) relative to wild-type controls (G, I, K). Hoechst counterstaining is shown in blue. (M) Comparison of percentages of Brn3+ cells in the total population of retinal cells between Chx10Cre-RBPf/fand wild-type retinae (*P < 0.05, **P < 0.01). Scale bars, 100 μm.