Peripheral inflammation triggers long-term increases in NMDA NR2B receptor mediated currents in cingulate pyramidal cells. NR2B sensitive component of NMDA receptor mediated EPSCs were enhanced in mice with CFA injection. a. Traces of EPSCs show the currents at different time points during application of drugs. Ro 25–6981 produced its maximal effect at 3 min after bath application, and a higher dose of Ro 25–6981 (3 μM) had no additional effects. The remaining currents can be totally blocked by AP-5 (50 μM). b. A selective NR2B antagonist, Ro 25–6981, partially inhibited NMDA receptor-mediated EPSCs. The time course of changes in EPSC amplitude before and during the application of Ro 25–6981 (0.3 and 3 μM) and AP-5 (50 μM) in ACC neurons from both saline (control, open symbols) and CFA-injected (filled sumbols) mice is shown. Modified from Wu et al. (2005).