Figure 6

Reduction in LV size and increased neural progenitors proliferation in the SVZ of Tdo^-/- mice. (A) Paraffin-embedded coronal brain sections (5 μm) were stained with H&E, and the size of each ventricle was measured. The boxed area in the subventricular zone (SVZ) was used for studies in Figures 6C-F. Arrows illustrate the change in the size of the LV in Tdo^-/- mice. (B) Quantitative size of each ventricle in both genotypes. Relative mean size in each ventricle of Tdo^+/+ mice was defined as 100%. Results are expressed as the means ± S.E. and tested for significance with ANOVA and Scheffe's post hoc test (p < 0.05). (C-F) Incorporation of BrdU in neural progenitors of SVZ of Tdo^+/+ and Tdo^-/- mice. Frozen coronal sections (20 μm) of the SVZ of the brain of 13-week-old mice were stained with TO-PRO-3 (nuclei, Ca and Cb), BrdU (Cc-f, Dc-f, E, and F), DCX (E), PSA-NCAM (F), nestin (Da, Db, De and Df) (boxed area in Figure 6A) at 24 h after BrdU injection. Inset, a merged view at high magnification (Df) of BrdU (green) and nestin (red). Ctx, cortex; Str, striatum; Sep, septum; Hip, hippocampus; D3V, dorsal 3rd ventricle; Aq, Aqueduct; and CC, corpus callosum. Bars: 100 μm.