Figure 3
From: Rapid and Direct Transport of Cell Surface APP to the Lysosome defines a novel selective pathway
Quantitation of APP enrichment in transfected cells and primary neurons. SN56 cells (A-B) were transfected with transfected with APP-CFP (green channel) and either LAMP1-mRFP or Cox8-mRFP (red channel) and imaged by confocal microscopy. Mouse neurons (C) were immunstained with Anti-C-terminal APP (green channel) and Anti-LAMP1 (red channel). The co-localization of the green and red channels can be view qualitatively in the yellow (merge) images and the histograms of these images are shown. To quantify colocalization, the brightest 2% of green (to the right of the dotted yellow line) and red pixels (above the yellow solid line on the histograms) is selected. The colocalized pixels are indicated in white in the Colocalized Pixels panels.