Figure 5
From: Rapid and Direct Transport of Cell Surface APP to the Lysosome defines a novel selective pathway
APP is enriched in the lysosome in cultured mouse neurons. A. Cultured mouse neurons were fixed and immunostained with antibodies against APP (green) or compartment markers (red) and nuclei were counterstained with Hoechst dye (blue). Images were acquired using laser scanning confocal microscopy demonstrating the green, red and merged image channels. An additional channel was generated showing the where the brightest 2% of red and green pixels are colocalized (See Figure 3). Scale bar = 5 microns. B. Quantitation of colocalization of the brightest 2% of pixels of APP and compartment makers. Each bar represents with mean ± SEM of at least 3 independent immunostaining experiments, imaging total of at least 25 cells. * indicates the LAMP1 staining is significantly different from Rab5 or Rab9 (P < 0.05).