Figure 6
From: Rapid and Direct Transport of Cell Surface APP to the Lysosome defines a novel selective pathway
London and Swedish Mutations reduce APP levels in late endosomal and lysosomal compartments. SN56 cells were transiently co-transfected with the βAPP-CFP construct with or without the London or the Swedish mutant along with compartment markers. Representative fixed cells were imaged using laser-scanning confocal microscopy. The colocalization of the brightest 2% of pixels of APP mutants with Rab5 (A), Rab9 (B) and LAMP1 (C) was quantitated using Imaris software. Values are expressed as the mean ± SEM for a minimum of 50 cells drawn from at least 4 independent transfections. * indicates statistical difference from wild type and all other compartment markers (p < 0.05). ** indicates compartment markers that are statistically different from wild type (p < 0.05) but not from each other. Scale bar = 10 microns.