Figure 8
From: Rapid and Direct Transport of Cell Surface APP to the Lysosome defines a novel selective pathway
APP internalization into the endosomal/lysosomal system in SN56 cells. A. Representative images of the uptake of anti-HA antibody or fluorescent dextran (green) to lysosomes labeled with LAMP1-mRFP (red). B. Internalization of surface labeled APP was quantitated by determining the co-localization of the brightest 2% of pixels with fluorescent-tagged compartment marker proteins. Each point represents the Mean ± SEM for at least 3 independent experiments examining 4 representative cells each. These means were compared statistically using and ANOVA at each time point. * indicates statistical significance between LAMP1 and all other markers (P < 0.05). ** indicates Rab5 is statistically difference from Rab9 (P < 0.05).