Early-onset neurological dysfunction and shortened lifespan of A53T mice. (A) Open-field tests reveal a significant decline of spontaneous ambulatory activities in A53T mice as compared to littermate controls at 1 and 2 months of age. **p < 0.01, ***p < 0.001. (B) Both fore and hind limb grip strength of A53T mice are impaired compared to littermate controls at 2 months of age. *p < 0.05, **p < 0.01. (C) Line graph reveals the onset of paralysis observed in A53T mice (n = 20). The average age of onset was 71.6 ± 2.0 days. (D) Line graph depicts the survival rate of A53T mice (n = 7). The average lifespan of the A53T mice was 90.4 ± 2.9 days.