Activation of microglia in A53T mice. (A-C) Representative images show Iba1 staining in cortex (Ctx), brainstem (BS) and substance nigra pars compacta (SNc) sections derived from A53T and nTg littermate control mice. Scale bars: 50 μm (A), 200 μm (B-C). (D-E), Quantitative measurement of the microglia (Iba1 positive cells) and microglia clusters in the cortex, substantia nigra pars compacta and brainstem (n = 3 per genotype). **p < 0.01, ***p < 0.001, compared with nTg; ##p < 0.01, compared with cortex.