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Figure 5 | Molecular Brain

Figure 5

From: Iron content of ferritin modulates its uptake by intestinal epithelium: implications for co-transport of prions

Figure 5

Iron content of ferritin influences its uptake by duodenal enterocytes. Immunostaining of 3 wild type mice fed -DFO and +DFO treated scrapie infected mouse brain homogenate with antibody to ZO-1 followed by anti-mouse-TRITC secondary antibody shows intact tight junctions (panels 2 and 4-6, arrow). Other sections do not show the epithelial cell layer and lack ZO-1 reactivity (panels 1 and 3). Reaction with anti-ferritin antibody followed by anti-rabbit-FITC shows more uptake of -DFO ferritin compared to the +DFO sample (compare panels 1-3 with 4-6, arrow-heads). Most ferritin from -DFO sample accumulates in intestinal crypts after 4 hours of chase (panels 1 and 3). Three representative sections are shown from a total of 6 different experiments performed by three different individuals. Bar: 10 μm. Insets show enlarged image of boxed areas.

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Molecular Brain

ISSN: 1756-6606