Figure 1From: CaMKIV over-expression boosts cortical 4-7 Hz oscillations during learning and 1-4 Hz delta oscillations during sleepExperimental design. To examine behavioral states and freezing behavior, both WT and CaMKIV over-expressed mice were chronically instrumented with frontal-parietal EEG and EMG recording electrodes. Sleep and wakefulness recordings were obtained on the pre-conditioning day. Following the pre-conditioning day, between 7 and 8 AM, mice were trace fear conditioned. Mice were then returned to their home cage where their post-conditioning sleep was recorded. Trace fear conditioning was conducted for 45 minutes in a shock chamber. This paradigm involved repeated trials of a presentation of a tone, followed by an interval (trace interval) and subsequently by a foot shock. Trace fear testing was conducted the following day in a similar fashion, only in this case, the shock stimulus was omitted.Back to article page