Figure 5

CaMKIV over-expression enhances 4-7.5 Hz waves which can localize to the prelimbic cortex. (A) Grouped data showing that WT and CaMKIV over-expressed mice have significant enhancements in 4-7.5 Hz power, during the trace fear interval, throughout the conditioning protocol (* is p < 0.05 compared to baseline). This effect mirrored the learning-curve for both mice. However, the magnitude of the effect was significantly greater in CaMKIV over-expressed mice as compared to WT for the conditioning phase (# is p < 0.05). (B) Example EEG activity corresponding to panel A, which was band-pass filtered for visual inspection of 4-7.5 Hz power. Four-7.5 Hz activity (50 μV scale bar) occurred during periods of freezing behavior and appeared to be more frequent and often larger in CaMKIV over-expressed mice. (C) Trial by trial changes in freezing during the trace interval were positively correlated to changes in 4-7.5 Hz power during conditioning. Dashed lines are 95% confidence intervals. (D) When WT mice are trained with a 15s trace fear interval, prominent 4-7.5 Hz power can be localized to the prelimbic cortex, during conditioning (* is p < 0.05 compared to baseline) but not on the testing day. (E) Trace fear conditioning (15s trace interval) learning curve for WT mice (* is p < 0.05 compared to baseline). Notice the memory enhancement when compared to Fig. 3A. BL is baseline, and values are means +/- SEM.