Expression and localization of GGT in the rat cerebellum. A) Lysates of HEK293 cells transfected with HA-GGTβ or vehicle plasmid were subjected to immunoblotting (IB) with anti-HA or -GGTβ antibody at indicated dilution. B) Homogenates of cerebellum from rats at indicated ages were subjected to IB with indicated antibodies. C) P10 rat cerebellar sections were stained with antibody against GGTα or control antibody, together with MAP2. Hoechst was used to mark cell layers. ML: molecular layer; WM: white matter. Scale bar is 200 μm (left and right lanes) or 20 μm (middle lane). D) P15 rat cerebellar sections were stained with antibody against GGTβ or control antibody, together with Calbindin. DAPI was used to mark cell layers. Scale bar is 20 μm.