Figure 3

Inhibition of GGT impedes dendrite development of PCs. P11 rat cerebellar slices at DIV2 were transfected with pCAG-EYFP plasmid. At DIV3, cultured slices were treated with DMSO or GGTi-2147 (2.5 μM) for additional 3 days. A) Representative images of PCs at DIV5, after treatment with DMSO or GGTi-2147. B) Quantification for the number of crossings. C) Quantification for total dendritic length between neighboring circles at indicated distance from soma. Data are shown as means ± SEM (n = 31 for control; n = 31 for GGTi-2147). *P < 0.05, **P < 0.01, ***P < 0.001; Student's t test. Scale bar = 20 μm.